Web9 de nov. de 2024 · As an important part of phosphate transporters (PHT), high-affinity phosphate transporters increase the P i influx capacity by several folds at low (μM) concentrations, which are considered as the main mechanism and compensation for P i deficiency (Lee 1993; Ming et al. 2006 ). Webcerevisiae mutant, PAM2, that lacks high-affinity phosphate transporters (Martinez and Persson 1998) was transformed with the coding region of LjPT3 linked to a constitutive promoter.
Expression analysis of three phosphate transporter genes in
WebPit is a low-affinity, high-velocity phosphate absorption system that relies on proton motive force to generate ATP, whereas Pst is an ABC transporter with ATP-driven high-affinity phosphate uptake . The Pst system is generally composed of PstS, PstC, PstA, PstB and … Web24 de out. de 2003 · Although high affinity transporters contribute to zinc, copper, and iron uptake when these metals are present at moderate levels ( 24, 25, 26 ), upon exposure to elevated concentrations of these metals the low affinity transporter (s) become the primary source of metal for the cell ( 27, 28, 29, 30 ). optima over the counter coverage
Increased expression of OsPT1, a high-affinity phosphate …
Webregulate low- and high-affinity phosphate transport. In this manner, the interplay of positive and negative feedback loops leads to the bistability of phosphate transporter usage. Cells express predominantly either low- or high-affinity transporters, both of which have similar phosphate uptake capacities (Wykoff et al., 2007). WebMethods Summary The Piriformospora indica high affinity phosphate transporter PiPT (accession number A8N031) was expressed in Saccharomyces cerevisiae and purified using a polyhistidine affinity-tag. Solubilization and purification used dodecyl-beta-D-maltoside followed by n- nonyl-beta-D-glucoside. Web19 de dez. de 2024 · Phosphate (Pi) transporters in plants mediate phosphorus (P) acquisition, translocation and recycling. In this study, a putative high-affinity Pi transporter gene ( CoPht1;3, GenBank KF989483.1) was isolated and characterised from oil-tea tree ( Camellia oleifera Abel .). optima overnight shipping