Immortalized human podocyte cell line

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August undefined, 2024

WitrynaImmortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T … Witryna17 wrz 2024 · Generally, conditionally immortalized podocytes have lost many of their phenotypes in vivo, including process formation at high cell density with conventional …

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WitrynaOur normal human primary cells retain in vivo physiologies and custom lots are available from a diverse donor pool. ATCC hTERT-immortalized primary cells also display … Witryna1 mar 2010 · Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term urinary cell … can rats climb bird feeder poles

Podocyte culture: Tricks of the trade - NI - Wiley Online …

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … WitrynaLoss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled bran… WitrynaCell Lines. HK2 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM LG) supplemented with 10% foetal bovine serum (FBS) (Euroclone S.p.A., Pero, MI, Italy). Immortalized human podocytes, previously characterized were cultured in DMEM High Glucose (Euroclone) with 10% FBS. Glomerular endothelial … can rats climb chicken wire

(PDF) A Conditionally Immortalized Human Podocyte Cell Line ...

Conditionally immortalized human podocyte cell lines …

WitrynaImmortalized Human Kidney Podocytes (IM-HKP) provided by Innoprot have been developed by immortalizing primary human healthy kidney podocytes with SV40 … WitrynaThe podocyte is a key cell in the glomerulus in health and disease: until recently, human podocytes have been difficult to study in vitro. We have developed a conditionally immortalized human podocyte cell line transfected with a temperature-sensitive simian virus 40 transgene: when the transgene is inactivated in vitro, these … flanderscompany.comWitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … flanders clinic

"WitrynaExperimental approach: Expression of TRPV1-4 channels was investigated at protein (immunocytochemistry, Western blot) and mRNA (Q-PCR) level in a conditionally immortalized human podocyte cell line. Channel function was assessed by measuring intracellular Ca 2+ concentration using Flou-4 Ca 2+ -indicator dye and patch clamp … " - Immortalized human podocyte cell line

Immortalized human podocyte cell line

Human Podocyte Cell Line (CIHP-1) Cell Lines - Ximbio

Witryna1 kwi 2002 · The conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [8, 40]. Podocytes were cultured in the medium which contained ... WitrynaTo investigate changes on the cellular level, we used an immortalized human podocyte cell line and generated a stable knockdown of Sphk2 (Sphk2-kd) by a lentiviral transduction method.

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Witryna14 lut 2015 · Mouse podocytes immortalised by transduction with a temperature sensitive large T antigen SV40 construct as previously described 45, 46, were used … Witryna1 mar 2010 · Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and …

Witryna17 maj 2012 · Conditionally immortalized human podocyte cell lines have been developed by transfection using both the temperature-sensitive mutant U19tsA58 of … Witryna1 mar 2010 · The generation of podocytes cell lines from urine of patients and healthy volunteers is novel and will help to advance studies of podocyte cell biology, and further improvements in the approaches to cell transformation and/or cell culture techniques are needed to allow cultured podocytes to fully reproduce in vivo characteristics. …

Witryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When … WitrynaThe Human Podocyte Cell Line (CIHP-1) is a primary cell line isolated from human kidney. Podocytes are highly specialised, terminally differentiated cells with a complex …

WitrynaG.J. Audesirk, in Comprehensive Toxicology, 2010 13.24.2.3.5 Immortal cell lines. Immortal cell lines are an alternative to primary cultures. Frequently used cell lines …

Witryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher … can rats climb glassWitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein … flanders clanWitrynaHuman cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug … flanders cleaning flanders cold centerWitrynaThese results suggest that podocyte cell lines can be established consistently from human urine. The generation of podocyte cell lines from urine of patients and … flanders commercial filtersWitryna18 sie 2024 · A conditionally immortalized murine podocyte cell line (E11) was obtained from Cell Line Services. Podocytes were propagated at 33 °C in RPMI1640 supplemented with 10% FBS and 10 U/mL of IFN-γ (Saint Louis, MO, USA) to enhance expression of thermosensitive T antigen. ... Shimada, H.; Rajagopalan, L.E. Rho … flanders clear by silver cityWitrynaSteroid-resistant nephrotic syndrome (SRNS) is a frequent cause of CKD. The discovery of monogenic causes of SRNS has revealed specific pathogenetic pathways, but these monogenic causes do not explain all cases of SRNS. To identify novel monogenic flanders christ is watching