Pmon530
WebJan 14, 2010 · The resulting plasmid pMON530-CLS was first introduced into Agrobacterium tumefaciens by electroporation and then transformed into the Arabidopsis las mutant by floral dip method (Clough and Bent 1998). Transgenic plants were selected on MS medium containing 20 mg/L Kanamycin. DNA Isolation and Gel Blot Analysis WebDownload scientific diagram Schematic representation of the T-DNA region of the binary vectors pMON337-TGMV-GFP and pMON530-GFP.
Pmon530
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WebMar 1, 2024 · This was then ligated into the pMON530-GFP vector, in frame with green fluorescent protein (GFP) under the control of the CaMV 35S promoter. The resultant pMON530-TCM1-GFP plasmids were introduced into tobacco (Nicotiana tabacum L.) leaves and cocultured at 25°C for 2 d. In the meantime, pMON530-GFP empty vector was used … WebJan 1, 1987 · The resultant plasmid, pMON530 (Fig. 7), retains the properties of pMON505 and the 35 S-NOS expression cassette now contains a unique cleavage site for Sinai between the pro- moter and polyadenylation signals. Tables listing the major restriction endonuclease cleavage sites useful for cloning and analysis of clones and transformants …
Webfragment from the plasmid pMON530 in Escherichia coli strain NM294 was used as the probe for transformed tissue DNA. Plas-mid DNA was isolated from E. coli using an alkaline lysis method (Liszewski et al., 1989; Sambrook et al., 1989). PCR amplification of the nos fragment for probe preparation or for plant DNA analysis was carried out ac- WebNational Center for Biotechnology Information
WebNov 1, 2024 · The pMON530:CaMV35S:ASL4-GFP plasmid was introduced into epidermal tobacco cells using the Agrobacterium-mediated infection method, with empty GFP vector as control. ASL4 showed localization in the nucleus in addition to the cytoplasm (Fig. 4 B), consistent with the closest homologous gene SlSAP12 ( Solyc02g087210 ) in tomato … WebJan 1, 2024 · The full-length ORF of SmMYB1 (without the stop codon) was fused to a green fluorescent protein (GFP) gene under the control of the CaMV 35S promoter within a pMON530 vector to generate a SmMYB...
WebApr 27, 2024 · Then, the constructed vector and the pMON530 (used as the control) was transformed into the ASE strain and expressed in tobacco leaves, respectively. In the leaves of control vector transformed plant, the fluorescence of GFP was detected in the cytoplasm and nucleus (Figure Figure3 3). In contrast (to this), the fluorescence of SmWRKY1/GFP ...
WebMay 1, 2024 · The resultant pMON530:CaMV35S:TCD33-GFP plasmid was confirmed by sequencing and introduced into the Agrobacterium strain EHA105. The localization of TCD33 was investigated by transient expression assays of the GFP fusion protein in tobacco ( Nicotiana tabacum ) cells by laser scanning confocal microscopy (LSCM) as described … crochet how to decrease single crochetWebApr 9, 2015 · The pMON530:CaMV35S:ASL3-GFP plasmid was introduced into tobacco cells using Agrobacterium-mediated infection method. Meanwhile, empty GFP vector was used as a control. As a result, the green fluorescent signals of ASL3-GFP fusion protein perfectly overlapped with chloroplast autofluorescence in transformed tobacco mesophyll … buffalo wings in longwoodWebJan 1, 2024 · The pMON530:CaMV35-GFP vector was used as a control. The GFP fluorescence signal in the tobacco mesophyll cells transformed with the pMON530:CaMV35S:TSV3-GFP plasmid perfectly overlapped with the chloroplast autofluorescence ( Figure 5B ), while the empty GFP vector without a specific targeting … crochet how to switch yarnWebOct 2, 1992 · The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function. All Science Journal Classification (ASJC) codes crochet how to blockWebJul 30, 2024 · The PCR products were cloned into the pMON530-GFP vector, which was transformed into tobacco (Nicotiana tabacum) mesophyll cells through Agrobacterium-mediated infection. Meanwhile, empty pMON530-GFP vector was used as control. The analysis was carried out based on previously described method (Jiang et al. 2014). … buffalo wings lawsuitWebMay 15, 2006 · This DNA fragment was cloned into a T-vector (TaKaRa, Japan), verified by sequencing, and inserted into the plant transformation vector pMON530 (Monsanto, USA), downstream to the 35S promoter. crochet how to make a slip knotbuffalo wings invention